• Immunodeficiency Analysis
• Immunophenotyping
• Stem Cell Analysis
• Viability Determinations
• Terminal Deoxynucleotidyl Transferase Analysis
• Flow Cytrometry Research

Flow cytometric analysis is a quantitative technique for measuring multiple cell parameters, including cell surface antigens, intracellular/nuclear antigens, and DNA content. Flow cytometry uses a beam of laser light which is projected through a liquid stream. The stream cells and other particles that when hit by a beam of light give out signals which detectors pick up. The signals provide significant information about various cellular properties present in the liquid thus assisting in diagnosis and therapy of patients.Analyses can be performed on peripheral blood, bone marrow, and/or solid tissue. The technique is most commonly used to assess immunologic status and oncology. Laboratory services include lymphocyte immunophenotyping to monitor the immune status of HIV-infected persons; cellular phenotypes including cell surface, cytoplasmic, and nuclear markers that are cell-lineage specific used in the classification of leukemias and lymphomas; and DNA content and cell cycle analysis. Lymphoproliferative/leukemic panels are important in identifying large granular lymphocytosis, follicular lymphoma, hairy cell leukemia, B-cell CLL, mantle cell lymphoma. Reflex testing to molecular genetics for confirming inherent molecular endpoints can be extremely beneficial in further classification of disease.

Sheri Yates, CLS, BS, MT(ASCP)

Flow Cytometry Supervisor